Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
POPX1, PP2CH, caMKN, CAMKPN, CaMKP-N, PPM1E
This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene.
WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human, ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Cytoskeleton,Microfilaments.
Western blot analysis of various lysates using PPM1E Rabbit mAb (A9374) at 1:1000 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180 s.
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