HRP-conjugated Mouse anti His-Tag mAb

Produktübersicht

• Artikelname: HRP-conjugated Mouse anti His-Tag mAb
• Artikelnummer: ABB-AE028
• Hersteller Artikelnummer: AE028
• Alternativnummer: ABB-AE028-100UL,ABB-AE028-1000UL,ABB-AE028-200UL,ABB-AE028-50UL,ABB-AE028-500UL
• Hersteller: ABclonal
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: All
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: HRP
• Alternative Synonym: His,His tag,His-tag

Produktbeschreibung

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [AMC0484]
• Reinheit: Affinity purification
• Sequenz: HHHHHH
• Target-Kategorie: His
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Species independent

Bilder

Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein,using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.