HRP-conjugated Mouse anti His-Tag mAb

Product Overview

• Article Name: HRP-conjugated Mouse anti His-Tag mAb
• Biozol Catalog Number: ABB-AE028
• Supplier Catalog Number: AE028
• Alternative Catalog Number: ABB-AE028-100UL,ABB-AE028-1000UL,ABB-AE028-200UL,ABB-AE028-50UL,ABB-AE028-500UL
• Manufacturer: ABclonal
• Host: Mouse
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: All
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: HRP
• Alternative Names: His,His tag,His-tag

Product Description

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [AMC0484]
• Purity: Affinity purification
• Sequence: HHHHHH
• Target: His
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Species independent

Images

Western blot analysis of extracts of normal 293T cells and 293T transfected with His-NAA20 protein,using HRP-conjugated Mouse anti His-Tag mAb (AE028) at 1:20000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.