Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0044
• Hersteller Artikelnummer: AP0044
• Alternativnummer: ABB-AP0044-100UL, ABB-AP0044-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: IGF1R/INSR, Phospho-INSR-Y1150/IGF1R-Y1135

Produktbeschreibung

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Produkteigenschaften

• Molekulargewicht: 154kDa/155kDa/156kDa
• NCBI: 3643
• UniProt: P06213
• Reinheit: Affinity purification
• Target-Kategorie: IGF1R/INSR

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Bilder

Western blot analysis of lysates from 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb (AP0044) at 1:1000 dilution. 293 cells were treated by EGF (25µg/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.