Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0044
• Supplier Catalog Number: AP0044
• Alternative Catalog Number: ABB-AP0044-100UL, ABB-AP0044-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: IGF1R/INSR, Phospho-INSR-Y1150/IGF1R-Y1135

Product Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Product Properties

• Molecular Weight: 154kDa/155kDa/156kDa
• NCBI: 3643
• UniProt: P06213
• Purity: Affinity purification
• Target: IGF1R/INSR

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Images

Western blot analysis of lysates from 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 Rabbit pAb (AP0044) at 1:1000 dilution. 293 cells were treated by EGF (25µg/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.