Phospho-RB-S811 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-RB-S811 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0089
• Hersteller Artikelnummer: AP0089
• Alternativnummer: ABB-AP0089-100UL, ABB-AP0089-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1, Phospho-RB-S811

Produktbeschreibung

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Produkteigenschaften

• Molekulargewicht: 106kDa
• NCBI: 5925
• UniProt: P06400
• Reinheit: Affinity purification
• Target-Kategorie: RB1

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,DNA Damage Repair,Protein phosphorylation,Cancer,Tumor suppressors,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G1 S Checkpoint.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-RB-S811 Rabbit pAb (AP0089) at 1:1000 dilution. HeLa cells were treated with UV for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.