Phospho-RB-S811 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-RB-S811 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0089
• Supplier Catalog Number: AP0089
• Alternative Catalog Number: ABB-AP0089-100UL, ABB-AP0089-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1, Phospho-RB-S811

Product Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Product Properties

• Molecular Weight: 106kDa
• NCBI: 5925
• UniProt: P06400
• Purity: Affinity purification
• Target: RB1

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,DNA Damage Repair,Protein phosphorylation,Cancer,Tumor suppressors,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G1 S Checkpoint.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-RB-S811 Rabbit pAb (AP0089) at 1:1000 dilution. HeLa cells were treated with UV for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.