Phospho-alpha-Synuclein-Y133 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-alpha-Synuclein-Y133 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0451
• Hersteller Artikelnummer: AP0451
• Alternativnummer: ABB-AP0451-20UL, ABB-AP0451-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PD1, NACP, PARK1, PARK4, Phospho-alpha-Synuclein-Y133

Produktbeschreibung

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimers disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.

Produkteigenschaften

• Molekulargewicht: 14kDa
• NCBI: 6622
• UniProt: P37840
• Reinheit: Affinity purification
• Target-Kategorie: SNCA

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cell Biology Developmental Biology,Apoptosis,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Dopamine Signaling in Parkinsons Disease,Neurodegenerative Diseases Markers.

Bilder

Western blot analysis of various lysates, using Phospho-alpha-Synuclein-Y133 pAb (AP0451) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated with Pervanadate (1 mM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.