Phospho-EIF4B-S422 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-EIF4B-S422 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0775
• Hersteller Artikelnummer: AP0775
• Alternativnummer: ABB-AP0775-20UL, ABB-AP0775-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: EIF-4B, PRO1843, Phospho-EIF4B-S422

Produktbeschreibung

Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.

Produkteigenschaften

• Molekulargewicht: 69kDa
• NCBI: 1975
• UniProt: P23588
• Reinheit: Affinity purification
• Target-Kategorie: EIF4B

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway.

Bilder

Western blot analysis of lysates from HeLa cells, using Phospho-EIF4B-S422 Rabbit pAb (AP0775) at 1:2000 dilution. HeLa cells were treated with EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.