Phospho-EIF4B-S422 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-EIF4B-S422 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0775
• Supplier Catalog Number: AP0775
• Alternative Catalog Number: ABB-AP0775-20UL, ABB-AP0775-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: EIF-4B, PRO1843, Phospho-EIF4B-S422

Product Description

Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.

Product Properties

• Molecular Weight: 69kDa
• NCBI: 1975
• UniProt: P23588
• Purity: Affinity purification
• Target: EIF4B

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-EIF4B-S422 Rabbit pAb (AP0775) at 1:2000 dilution. HeLa cells were treated with EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.