Phospho-NPM1-T199 Rabbit pAb, Unconjugated
Artikelnummer:
ABB-AP0836
- Bilder (1)
| Artikelname: | Phospho-NPM1-T199 Rabbit pAb, Unconjugated |
| Artikelnummer: | ABB-AP0836 |
| Hersteller Artikelnummer: | AP0836 |
| Alternativnummer: | ABB-AP0836-100UL,ABB-AP0836-20UL,ABB-AP0836-500UL,ABB-AP0836-1000UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | B23, NPM, Phospho-NPM1-T199 |
| The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. |
| Application Verdünnung: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Cell Biology Developmental Biology,Cell Cycle,Centrosome. |
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Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (AP0836) at 1:900 dilution. HeLa cells were treated with nocodazole (50 ng/ml) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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