Phospho-NPM1-T199 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP0836
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| Article Name: | Phospho-NPM1-T199 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP0836 |
| Supplier Catalog Number: | AP0836 |
| Alternative Catalog Number: | ABB-AP0836-100UL,ABB-AP0836-20UL,ABB-AP0836-500UL,ABB-AP0836-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | B23, NPM, Phospho-NPM1-T199 |
| The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Cell Biology Developmental Biology,Cell Cycle,Centrosome. |
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Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (AP0836) at 1:900 dilution. HeLa cells were treated with nocodazole (50 ng/ml) at 37°C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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