Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
CYP, SRCyp, SCAF10, CARS-Cyp, Phospho-PPIG-S376
Enables cyclosporin A binding activity and peptidyl-prolyl cis-trans isomerase activity. Involved in protein peptidyl-prolyl isomerization. Located in cytosol and nuclear speck.
Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (AP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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