Phospho-PPIG-S376 Rabbit pAb, Unconjugated

Artikelnummer: ABB-AP0842
Artikelname: Phospho-PPIG-S376 Rabbit pAb, Unconjugated
Artikelnummer: ABB-AP0842
Hersteller Artikelnummer: AP0842
Alternativnummer: ABB-AP0842-100UL, ABB-AP0842-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: CYP, SRCyp, SCAF10, CARS-Cyp, Phospho-PPIG-S376
Enables cyclosporin A binding activity and peptidyl-prolyl cis-trans isomerase activity. Involved in protein peptidyl-prolyl isomerization. Located in cytosol and nuclear speck.
Molekulargewicht: 89kDa
NCBI: 9360
UniProt: Q13427
Reinheit: Affinity purification
Target-Kategorie: PPIG
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Signal Transduction.
Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (AP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.