Phospho-PPIG-S376 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PPIG-S376 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0842
• Supplier Catalog Number: AP0842
• Alternative Catalog Number: ABB-AP0842-100UL, ABB-AP0842-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CYP, SRCyp, SCAF10, CARS-Cyp, Phospho-PPIG-S376

Product Description

Enables cyclosporin A binding activity and peptidyl-prolyl cis-trans isomerase activity. Involved in protein peptidyl-prolyl isomerization. Located in cytosol and nuclear speck.

Product Properties

• Molecular Weight: 89kDa
• NCBI: 9360
• UniProt: Q13427
• Purity: Affinity purification
• Target: PPIG

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Signal Transduction.

Images

Western blot analysis of various lysates using Phospho-PPIG-S376 Rabbit pAb (AP0842) at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. C2C12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.