Phospho-HSL-S563 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-HSL-S563 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0851
• Hersteller Artikelnummer: AP0851
• Alternativnummer: ABB-AP0851-20UL, ABB-AP0851-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: HSL, LHS, REH, AOMS4, FPLD6, Phospho-HSL-S563

Produktbeschreibung

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Produkteigenschaften

• Molekulargewicht: 117kDa
• NCBI: 3991
• UniProt: Q05469
• Reinheit: Affinity purification
• Target-Kategorie: LIPE

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Cardiovascular,Lipids.

Bilder

Western blot analysis of lysates from Mouse brown fat using Phospho-HSL-S563 Rabbit pAb (AP0851) at 1:5000 dilutionincubated overnight at 4°C. Mouse brown fat were treated by lambda-PP mixed solution (1 µl) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.