Phospho-HSL-S563 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-HSL-S563 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0851
• Supplier Catalog Number: AP0851
• Alternative Catalog Number: ABB-AP0851-20UL, ABB-AP0851-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HSL, LHS, REH, AOMS4, FPLD6, Phospho-HSL-S563

Product Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Product Properties

• Molecular Weight: 117kDa
• NCBI: 3991
• UniProt: Q05469
• Purity: Affinity purification
• Target: LIPE

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Cardiovascular,Lipids.

Images

Western blot analysis of lysates from Mouse brown fat using Phospho-HSL-S563 Rabbit pAb (AP0851) at 1:5000 dilutionincubated overnight at 4°C. Mouse brown fat were treated by lambda-PP mixed solution (1 µl) at 30°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.