Phospho-FOXO3A-S294 Rabbit pAb, Unconjugated

Artikelnummer: ABB-AP0856
Artikelname: Phospho-FOXO3A-S294 Rabbit pAb, Unconjugated
Artikelnummer: ABB-AP0856
Hersteller Artikelnummer: AP0856
Alternativnummer: ABB-AP0856-20UL,ABB-AP0856-100UL,ABB-AP0856-500UL,ABB-AP0856-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Phospho-FOXO3A-S294
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Klonalität: Polyclonal
Molekulargewicht: 71kDa
NCBI: 2309
UniProt: O43524
Reinheit: Affinity purification
Sequenz: PGSPT
Target-Kategorie: FOXO3
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (AP0856) at 1:1000 dilution. NIH/3T3 cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.