Phospho-FOXO3A-S294 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-FOXO3A-S294 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0856
• Supplier Catalog Number: AP0856
• Alternative Catalog Number: ABB-AP0856-20UL,ABB-AP0856-100UL,ABB-AP0856-500UL,ABB-AP0856-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Phospho-FOXO3A-S294

Product Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 71kDa
• NCBI: 2309
• UniProt: O43524
• Purity: Affinity purification
• Sequence: PGSPT
• Target: FOXO3
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Autophagy,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (AP0856) at 1:1000 dilution. NIH/3T3 cells were treated with 10% FBS at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.