Phospho-IRF3-S386 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-IRF3-S386 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0857
• Hersteller Artikelnummer: AP0857
• Alternativnummer: ABB-AP0857-500UL,ABB-AP0857-100UL,ABB-AP0857-1000UL,ABB-AP0857-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: IIAE7, Phospho-IRF3-S386

Produktbeschreibung

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.

Produkteigenschaften

• Molekulargewicht: 47kDa
• NCBI: 3661
• UniProt: Q14653
• Reinheit: Affinity purification
• Sequenz: ASSLE
• Target-Kategorie: IRF3

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Immunology Inflammation,Cytokines,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Bilder

Western blot analysis of lysates from 293F cells using Phospho-IRF3-S386 Rabbit pAb (AP0857) at 1:1000 dilutionincubated overnight at 4°C. 293F cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.