Phospho-IRF3-S386 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-IRF3-S386 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0857
• Supplier Catalog Number: AP0857
• Alternative Catalog Number: ABB-AP0857-500UL,ABB-AP0857-100UL,ABB-AP0857-1000UL,ABB-AP0857-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: IIAE7, Phospho-IRF3-S386

Product Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.

Product Properties

• Molecular Weight: 47kDa
• NCBI: 3661
• UniProt: Q14653
• Purity: Affinity purification
• Sequence: ASSLE
• Target: IRF3

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Immunology Inflammation,Cytokines,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Images

Western blot analysis of lysates from 293F cells using Phospho-IRF3-S386 Rabbit pAb (AP0857) at 1:1000 dilutionincubated overnight at 4°C. 293F cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 µg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.