Phospho-Histone H4-S1 Rabbit pAb

Produktübersicht

• Artikelname: Phospho-Histone H4-S1 Rabbit pAb
• Artikelnummer: ABB-AP0901
• Hersteller Artikelnummer: AP0901
• Alternativnummer: ABB-AP0901-100UL,ABB-AP0901-20UL,ABB-AP0901-500UL,ABB-AP0901-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Synonym: H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, H4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, HIST1H4A, Phospho-Histone H4-S1

Produktbeschreibung

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 11kDa
• NCBI: 8359
• UniProt: P62805
• Reinheit: Affinity purification
• Sequenz: MSGR
• Target-Kategorie: H4C1
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted), ResearchArea: Protein phosphorylation,Cell Biology Developmental Biology,Cell Cycle.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (A1131). NIH/3T3 cells were treated with CIP(20uL/400ul) at 37°C for 1 hour or Nocodazole (50 ng/mL) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.