Phospho-Histone H4-S1 Rabbit pAb

Product Overview

• Article Name: Phospho-Histone H4-S1 Rabbit pAb
• Biozol Catalog Number: ABB-AP0901
• Supplier Catalog Number: AP0901
• Alternative Catalog Number: ABB-AP0901-100UL,ABB-AP0901-20UL,ABB-AP0901-500UL,ABB-AP0901-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, H4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, HIST1H4A, Phospho-Histone H4-S1

Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 11kDa
• NCBI: 8359
• UniProt: P62805
• Purity: Affinity purification
• Sequence: MSGR
• Target: H4C1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted), ResearchArea: Protein phosphorylation,Cell Biology Developmental Biology,Cell Cycle.

Images

Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (A1131). NIH/3T3 cells were treated with CIP(20uL/400ul) at 37°C for 1 hour or Nocodazole (50 ng/mL) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.