phospho-LATS1-S909/LATS2-S872 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: phospho-LATS1-S909/LATS2-S872 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0904
• Hersteller Artikelnummer: AP0904
• Alternativnummer: ABB-AP0904-100UL,ABB-AP0904-20UL,ABB-AP0904-1000UL,ABB-AP0904-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: LATS1, WARTS, wts, phospho-LATS1-S909/LATS2-S872

Produktbeschreibung

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 76kDa/126kDa
• NCBI: 9113
• UniProt: O95835
• Reinheit: Affinity purification
• Sequenz: AHSLV
• Target-Kategorie: LATS1/LATS2
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Protein phosphorylation,Protein phosphorylation.

Bilder

Western blot analysis of lysates from HeLa cells, using phospho-LATS1-S909/LATS2-S872 Rabbit pAb (AP0904) at 1:500 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.