phospho-LATS1-S909/LATS2-S872 Rabbit pAb, Unconjugated

Product Overview

• Article Name: phospho-LATS1-S909/LATS2-S872 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0904
• Supplier Catalog Number: AP0904
• Alternative Catalog Number: ABB-AP0904-100UL,ABB-AP0904-20UL,ABB-AP0904-1000UL,ABB-AP0904-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: LATS1, WARTS, wts, phospho-LATS1-S909/LATS2-S872

Product Description

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 76kDa/126kDa
• NCBI: 9113
• UniProt: O95835
• Purity: Affinity purification
• Sequence: AHSLV
• Target: LATS1/LATS2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Protein phosphorylation,Protein phosphorylation.

Images

Western blot analysis of lysates from HeLa cells, using phospho-LATS1-S909/LATS2-S872 Rabbit pAb (AP0904) at 1:500 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.