Synthetic peptide. This information is considered to be commercially sensitive.
Alternative Synonym:
PPP2CA/PPP2CB, Phospho-PPP2CA/PPP2CB-Y307
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human,Mouse
Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (AP0927) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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