Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb

Artikelnummer: ABB-AP0927
Artikelname: Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb
Artikelnummer: ABB-AP0927
Hersteller Artikelnummer: AP0927
Alternativnummer: ABB-AP0927-100UL,ABB-AP0927-20UL,ABB-AP0927-500UL,ABB-AP0927-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Alternative Synonym: PPP2CA/PPP2CB, Phospho-PPP2CA/PPP2CB-Y307
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Klonalität: Polyclonal
NCBI: 5515
UniProt: P67775
Reinheit: Affinity purification
Sequenz: PDYFL
Target-Kategorie: PPP2CA/PPP2CB
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse
Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (AP0927) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.