Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb

Product Overview

• Article Name: Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb
• Biozol Catalog Number: ABB-AP0927
• Supplier Catalog Number: AP0927
• Alternative Catalog Number: ABB-AP0927-100UL,ABB-AP0927-20UL,ABB-AP0927-500UL,ABB-AP0927-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: PPP2CA/PPP2CB, Phospho-PPP2CA/PPP2CB-Y307

Product Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]

Product Properties

• Clonality: Polyclonal
• NCBI: 5515
• UniProt: P67775
• Purity: Affinity purification
• Sequence: PDYFL
• Target: PPP2CA/PPP2CB
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse

Images

Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (AP0927) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.