Phospho-PLN-S16/T17 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-PLN-S16/T17 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP0956
• Hersteller Artikelnummer: AP0956
• Alternativnummer: ABB-AP0956-100UL,ABB-AP0956-20UL,ABB-AP0956-1000UL,ABB-AP0956-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: PLB, CMD1P, CMH18, Phospho-PLN-S16/T17

Produktbeschreibung

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 6kDa
• NCBI: 5350
• UniProt: P26678
• Reinheit: Affinity purification
• Sequenz: ASTIE
• Target-Kategorie: PLN
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Neuroscience,Calcium Signaling,Cardiovascular,Heart,Contractility.

Bilder

Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (AP0956) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.