Phospho-PLN-S16/T17 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PLN-S16/T17 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0956
• Supplier Catalog Number: AP0956
• Alternative Catalog Number: ABB-AP0956-100UL,ABB-AP0956-20UL,ABB-AP0956-1000UL,ABB-AP0956-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PLB, CMD1P, CMH18, Phospho-PLN-S16/T17

Product Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 6kDa
• NCBI: 5350
• UniProt: P26678
• Purity: Affinity purification
• Sequence: ASTIE
• Target: PLN
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Neuroscience,Calcium Signaling,Cardiovascular,Heart,Contractility.

Images

Western blot analysis of various lysates using Phospho-PLN-S16/T17 Rabbit pAb (AP0956) at 1:1000 dilution. Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.