Phospho-SIRT1-S47 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-SIRT1-S47 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP0976
• Hersteller Artikelnummer: AP0976
• Alternativnummer: ABB-AP0976-100UL,ABB-AP0976-20UL,ABB-AP0976-500UL,ABB-AP0976-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: SIR2, SIR2L1, SIR2alpha, Phospho-SIRT1-S47

Produktbeschreibung

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1520]
• Molekulargewicht: 82kDa
• NCBI: 23411
• UniProt: Q96EB6
• Reinheit: Affinity purification
• Sequenz: ERSPG
• Target-Kategorie: SIRT1
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Mitochondrial metabolism,AMPK Signaling Pathway.

Bilder

Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (AP0976) at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.