Phospho-SIRT1-S47 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-SIRT1-S47 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP0976
• Supplier Catalog Number: AP0976
• Alternative Catalog Number: ABB-AP0976-100UL,ABB-AP0976-20UL,ABB-AP0976-500UL,ABB-AP0976-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: SIR2, SIR2L1, SIR2alpha, Phospho-SIRT1-S47

Product Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1520]
• Molecular Weight: 82kDa
• NCBI: 23411
• UniProt: Q96EB6
• Purity: Affinity purification
• Sequence: ERSPG
• Target: SIRT1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Mitochondrial metabolism,AMPK Signaling Pathway.

Images

Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (AP0976) at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.