Phospho-Bad-S112 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Bad-S112 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-AP1010
• Hersteller Artikelnummer: AP1010
• Alternativnummer: ABB-AP1010-20UL,ABB-AP1010-100UL,ABB-AP1010-500UL,ABB-AP1010-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: BBC2, BCL2L8, Phospho-Bad-S112

Produktbeschreibung

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC1555]
• Molekulargewicht: 18kDa
• NCBI: 572
• UniProt: Q92934
• Reinheit: Affinity purification
• Target-Kategorie: BAD
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (AP1010) at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight or treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.