Phospho-Bad-S112 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-Bad-S112 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1010
• Supplier Catalog Number: AP1010
• Alternative Catalog Number: ABB-AP1010-20UL,ABB-AP1010-100UL,ABB-AP1010-500UL,ABB-AP1010-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: BBC2, BCL2L8, Phospho-Bad-S112

Product Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1555]
• Molecular Weight: 18kDa
• NCBI: 572
• UniProt: Q92934
• Purity: Affinity purification
• Target: BAD
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (AP1010) at 1:1000 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight or treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.