Phospho-Bcl-2-S87 Rabbit pAb

Artikelnummer: ABB-AP1071
Artikelname: Phospho-Bcl-2-S87 Rabbit pAb
Artikelnummer: ABB-AP1071
Hersteller Artikelnummer: AP1071
Alternativnummer: ABB-AP1071-100UL,ABB-AP1071-20UL,ABB-AP1071-500UL,ABB-AP1071-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Alternative Synonym: Bcl-2, PPP1R50, Phospho-Bcl-2-S87
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Klonalität: Polyclonal
Molekulargewicht: 26kDa
NCBI: 596
UniProt: P10415
Reinheit: Affinity purification
Sequenz: ALSPV
Target-Kategorie: BCL2
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Invasion and Metastasis,Signal Transduction,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Autophagy,Death Receptor Signaling Pathway,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases,Stem Cells.
Western blot analysis of lysates from C6 cells, using Phospho-Bcl-2-S87 Rabbit pAb (AP1071) at 1:1000 dilution. C6 cells were treated with Paclitaxel (100 nM) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.