Phospho-HSL-S853 Rabbit mAb, Unconjugated

Artikelnummer: ABB-AP1151
Artikelname: Phospho-HSL-S853 Rabbit mAb, Unconjugated
Artikelnummer: ABB-AP1151
Hersteller Artikelnummer: AP1151
Alternativnummer: ABB-AP1151-100UL,ABB-AP1151-20UL,ABB-AP1151-1000UL,ABB-AP1151-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: HSL, LHS, REH, AOMS4, FPLD6, Phospho-HSL-S853
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC1657]
Molekulargewicht: 117kDa
NCBI: 3991
UniProt: Q05469
Reinheit: Affinity purification
Sequenz: RRSVS
Target-Kategorie: LIPE
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Cardiovascular,Lipids.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HSL-S853 Rabbit mAb (AP1151) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.