Phospho-HSL-S853 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1151
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| Article Name: | Phospho-HSL-S853 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1151 |
| Supplier Catalog Number: | AP1151 |
| Alternative Catalog Number: | ABB-AP1151-100UL,ABB-AP1151-20UL,ABB-AP1151-1000UL,ABB-AP1151-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HSL, LHS, REH, AOMS4, FPLD6, Phospho-HSL-S853 |
| The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Cardiovascular,Lipids. |
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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HSL-S853 Rabbit mAb (AP1151) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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