Phospho-c-Jun-T93 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-c-Jun-T93 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1218
• Hersteller Artikelnummer: AP1218
• Alternativnummer: ABB-AP1218-100UL, ABB-AP1218-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: AP1, p39, AP-1, cJUN, c-Jun, Phospho-c-Jun-T93

Produktbeschreibung

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Produkteigenschaften

• Molekulargewicht: 36kDa
• NCBI: 3725
• UniProt: P05412
• Reinheit: Affinity purification
• Target-Kategorie: JUN

Anwendungsbeschreibung

• Application Verdünnung: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-JNK Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Bilder

Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (AP1218) at 1:500 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Anisomycin (25 µg/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.