Phospho-c-Jun-T93 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-c-Jun-T93 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1218
• Supplier Catalog Number: AP1218
• Alternative Catalog Number: ABB-AP1218-100UL, ABB-AP1218-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: AP1, p39, AP-1, cJUN, c-Jun, Phospho-c-Jun-T93

Product Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Product Properties

• Molecular Weight: 36kDa
• NCBI: 3725
• UniProt: P05412
• Purity: Affinity purification
• Target: JUN

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-JNK Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Images

Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (AP1218) at 1:500 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Anisomycin (25 µg/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.