Phospho-EZH2/KMT6-T311 Rabbit pAb, Unconjugated

Artikelnummer: ABB-AP1269
Artikelname: Phospho-EZH2/KMT6-T311 Rabbit pAb, Unconjugated
Artikelnummer: ABB-AP1269
Hersteller Artikelnummer: AP1269
Alternativnummer: ABB-AP1269-20UL,ABB-AP1269-100UL,ABB-AP1269-1000UL,ABB-AP1269-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: WVS, ENX1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Phospho-EZH2/KMT6-T311
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Klonalität: Polyclonal
Molekulargewicht: 85kDa
NCBI: 2146
UniProt: Q15910
Reinheit: Affinity purification
Sequenz: KNTET
Target-Kategorie: EZH2
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Rat, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Chromatin Remodeling,Cancer,Tumor biomarkers.
Western blot analysis of lysates from C6 cells, using Phospho-EZH2/KMT6-T311 Rabbit pAb (AP1269) at 1:1000 dilution. C6 cells were treated with AICAR (0. 5 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.