Phospho-EZH2/KMT6-T311 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-EZH2/KMT6-T311 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1269
• Supplier Catalog Number: AP1269
• Alternative Catalog Number: ABB-AP1269-20UL,ABB-AP1269-100UL,ABB-AP1269-1000UL,ABB-AP1269-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: WVS, ENX1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Phospho-EZH2/KMT6-T311

Product Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 85kDa
• NCBI: 2146
• UniProt: Q15910
• Purity: Affinity purification
• Sequence: KNTET
• Target: EZH2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Rat, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Chromatin Remodeling,Cancer,Tumor biomarkers.

Images

Western blot analysis of lysates from C6 cells, using Phospho-EZH2/KMT6-T311 Rabbit pAb (AP1269) at 1:1000 dilution. C6 cells were treated with AICAR (0. 5 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.