Phospho-Ubiquitin-Ser65 Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-Ubiquitin-Ser65 Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1368
• Hersteller Artikelnummer: AP1368
• Alternativnummer: ABB-AP1368-20UL, ABB-AP1368-100UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: UBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80, L40, CEP52, RPL40, HUBCEP52, HEL-S-50, HMG20, Phospho-Ubiquitin-Ser65

Produktbeschreibung

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Produkteigenschaften

• Molekulargewicht: 18kDa/15kDa/77kDa
• NCBI: 6233
• UniProt: P62979
• Reinheit: Affinity purification
• Target-Kategorie: Ubiquitin

Anwendungsbeschreibung

• Application Verdünnung: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human

Bilder

Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368) at 1:400 dilution. PC-3 cells were treated with Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) at 37°C for 6 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.