Phospho-Ubiquitin-Ser65 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Ubiquitin-Ser65 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1368
• Supplier Catalog Number: AP1368
• Alternative Catalog Number: ABB-AP1368-20UL, ABB-AP1368-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: UBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80, L40, CEP52, RPL40, HUBCEP52, HEL-S-50, HMG20, Phospho-Ubiquitin-Ser65

Product Description

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

Product Properties

• Molecular Weight: 18kDa/15kDa/77kDa
• NCBI: 6233
• UniProt: P62979
• Purity: Affinity purification
• Target: Ubiquitin

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human

Images

Western blot analysis of lysates from PC-3 cells, using Phospho-Ubiquitin-Ser65 Rabbit pAb (AP1368) at 1:400 dilution. PC-3 cells were treated with Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) at 37°C for 6 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.