Phospho-GCN2-T899 Rabbit pAb, Unconjugated
Artikelnummer:
ABB-AP1428
- Bilder (1)
| Artikelname: | Phospho-GCN2-T899 Rabbit pAb, Unconjugated |
| Artikelnummer: | ABB-AP1428 |
| Hersteller Artikelnummer: | AP1428 |
| Alternativnummer: | ABB-AP1428-20UL,ABB-AP1428-100UL,ABB-AP1428-500UL,ABB-AP1428-1000UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | GCN2, PVOD2, Phospho-GCN2-T899 |
| This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. |
| Application Verdünnung: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Kinase,Serine threonine kinases. |
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Western blot analysis of lysates from HeLa cells, using Phospho-GCN2-T899 Rabbit pAb (AP1428) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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