Phospho-GCN2-T899 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-GCN2-T899 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1428
• Supplier Catalog Number: AP1428
• Alternative Catalog Number: ABB-AP1428-20UL,ABB-AP1428-100UL,ABB-AP1428-500UL,ABB-AP1428-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: GCN2, PVOD2, Phospho-GCN2-T899

Product Description

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 187kDa
• NCBI: 440275
• UniProt: Q9P2K8
• Purity: Affinity purification
• Sequence: HLTGM
• Target: EIF2AK4
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Kinase,Serine threonine kinases.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-GCN2-T899 Rabbit pAb (AP1428) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.