Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb, Unconjugated

Artikelnummer: ABB-AP1503
Artikelname: Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb, Unconjugated
Artikelnummer: ABB-AP1503
Hersteller Artikelnummer: AP1503
Alternativnummer: ABB-AP1503-100UL,ABB-AP1503-20UL,ABB-AP1503-1000UL,ABB-AP1503-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18/S19
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC62066]
Molekulargewicht: 20kDa
NCBI: 10398
UniProt: P24844
Reinheit: Affinity purification
Sequenz: RATSNV
Target-Kategorie: MYL9
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:2000 - 1:7000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins,Actins,TGF-b-Smad Signaling Pathway.
Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.