Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb, Unconjugated

Catalog Number: ABB-AP1503
Article Name: Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb, Unconjugated
Biozol Catalog Number: ABB-AP1503
Supplier Catalog Number: AP1503
Alternative Catalog Number: ABB-AP1503-100UL,ABB-AP1503-20UL,ABB-AP1503-1000UL,ABB-AP1503-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: LC20, MLC2, MRLC1, MYRL2, MLC-2C, MMIHS4, Phospho-Myosin Light Chain 2-T18/S19
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Clonality: Monoclonal
Clone Designation: [ARC62066]
Molecular Weight: 20kDa
NCBI: 10398
UniProt: P24844
Purity: Affinity purification
Sequence: RATSNV
Target: MYL9
Antibody Type: Primary Antibody
Application Dilute: WB,1:2000 - 1:7000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Motor Proteins,Actins,TGF-b-Smad Signaling Pathway.
Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18/S19 Rabbit mAb (AP1503) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.