Phospho-MRE11 (Ser676) Rabbit pAb, Unconjugated

Produktübersicht

• Artikelname: Phospho-MRE11 (Ser676) Rabbit pAb, Unconjugated
• Artikelnummer: ABB-AP1514
• Hersteller Artikelnummer: AP1514
• Alternativnummer: ABB-AP1514-20UL,ABB-AP1514-100UL,ABB-AP1514-1000UL,ABB-AP1514-500UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: ATLD, HNGS1, MRE11A, MRE11B, Phospho-MRE11 (Ser676)

Produktbeschreibung

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3 to 5 exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3 to 5 exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: WB
• Molekulargewicht: 81kDa
• NCBI: 4361
• UniProt: P49959
• Reinheit: Affinity purification
• Sequenz: IMSQS
• Target-Kategorie: Mre11
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000
• Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,ATM Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint,Immunology Inflammation.

Bilder

Western blot analysis of lysates from HeLa cells usingPhospho-MRE11 (Ser676) Rabbit pAb (AP1514) at1:800 dilution. HeLa cells were treated with Calyculin A (200 nM) at 37°C for 18 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:1s.