Phospho-MRE11 (Ser676) Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MRE11 (Ser676) Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1514
• Supplier Catalog Number: AP1514
• Alternative Catalog Number: ABB-AP1514-20UL,ABB-AP1514-100UL,ABB-AP1514-1000UL,ABB-AP1514-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ATLD, HNGS1, MRE11A, MRE11B, Phospho-MRE11 (Ser676)

Product Description

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3 to 5 exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3 to 5 exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Product Properties

• Clonality: Polyclonal
• Concentration: WB
• Molecular Weight: 81kDa
• NCBI: 4361
• UniProt: P49959
• Purity: Affinity purification
• Sequence: IMSQS
• Target: Mre11
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,ATM Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell Cycle Control-G2 M DNA Damage Checkpoint,Immunology Inflammation.

Images

Western blot analysis of lysates from HeLa cells usingPhospho-MRE11 (Ser676) Rabbit pAb (AP1514) at1:800 dilution. HeLa cells were treated with Calyculin A (200 nM) at 37°C for 18 hours after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:1s.