HRP conjugated Rabbit-Mouse IgM (Fc) mAb

Artikelnummer: ABB-AS094
Artikelname: HRP conjugated Rabbit-Mouse IgM (Fc) mAb
Artikelnummer: ABB-AS094
Hersteller Artikelnummer: AS094
Alternativnummer: ABB-AS094-100UL, ABB-AS094-1000UL, ABB-AS094-5X100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Mouse
Immunogen: This information is considered to be commercially sensitive.
Konjugation: HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Reinheit: Affinity purification
Target-Kategorie: Rabbit anti-Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: WB,1:10000 - 1:50000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Mouse
Western blot analysis of lysates from Mouse IgM using HRP Rabbit anti-Mouse IgM mAb (AS094) at 1:50000 dilution.
Lysates/proteins: 50ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.