HRP conjugated Rabbit-Mouse IgM (Fc) mAb

Catalog Number: ABB-AS094
Article Name: HRP conjugated Rabbit-Mouse IgM (Fc) mAb
Biozol Catalog Number: ABB-AS094
Supplier Catalog Number: AS094
Alternative Catalog Number: ABB-AS094-100UL, ABB-AS094-1000UL, ABB-AS094-5X100UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Mouse
Immunogen: This information is considered to be commercially sensitive.
Conjugation: HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purity: Affinity purification
Target: Rabbit anti-Mouse
Antibody Type: Secondary Antibody
Application Dilute: WB,1:10000 - 1:50000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Mouse
Western blot analysis of lysates from Mouse IgM using HRP Rabbit anti-Mouse IgM mAb (AS094) at 1:50000 dilution.
Lysates/proteins: 50ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.