HRP-conjugated Rabbit-Human IgE(Fc) PolymAb

Artikelnummer: ABB-AS161PM
Artikelname: HRP-conjugated Rabbit-Human IgE(Fc) PolymAb
Artikelnummer: ABB-AS161PM
Hersteller Artikelnummer: AS161PM
Alternativnummer: ABB-AS161PM-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: HRP
Alternative Synonym: Igh2, IGHE, IGHEP1, Human IgE
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock
Molekulargewicht: 47kDa
UniProt: P01854
Reinheit: Affinity purification
Target-Kategorie: IgE
Antibody Type: Secondary Antibody
Application Verdünnung: WB,1:80000 - 1:170000|ELISA, 1:5000-1:10000
Anwendungsbeschreibung: Cross-Reactivity: Human, ResearchArea: Adaptive immunity, Immunity.
Western blot analysis of various lysates using HRP-conjugated Rabbit-Human IgE(Fc) PolymAb (AS161PM) at 1:170000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Human IgG1,Human IgG2,Human IgG3,Human IgG4,Human IgA1,Human IgD,Human IgM,Human IgE
Exposure time: 90s.